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Harvard University Research Assistant I Lab in Cambridge, Massachusetts

53364BRAuto req ID:53364BRJob Code:403108 Research Assistant I Lab Location:USA - MA - Cambridge Business Title:Research Assistant I LabSub-Unit:------------ Salary Grade (https://hr.harvard.edu/salary-ranges#ranges) :052Time Status:Full-time Union:55 - Hvd Union Cler & Tech Workers Basic Qualifications:

Biological laboratory research experience is required.

Additional Qualifications and Skills:

B.A. or B.S. in Biological Sciences, Biochemistry, Chemistry, or related discipline is preferred. One to two years of laboratory experience that includes handling/screening of small animals (mice) is strongly preferred. Knowledge of cellular and molecular biology and the ability to apply this knowledge are required as are strong organizational and communication skills and ability to prioritize responsibilities.

Additional Information:

This is a one year grant funded term position with the possibility of extension based on availability of continued funding and satisfactory performance.

May be required to lift, move and transport related laboratory equipment. Required to work with a variety of hazardous materials under approved safety protocols. Required to work with and handle animals.

The Department of Stem Cell and Regenerative Biology and the FAS are committed to cultivating not only the diversity of our faculty, staff, and students but also in developing an inclusive culture (https://hr.fas.harvard.edu/inclusive-culture) that is vibrant, engaging and encouraging of innovation as well as intellectual debate. We believe creating and maintaining an inclusive workplace allows employees from all backgrounds and walks of life to achieve their fullest potential. We also believe an inclusive culture is one that accepts, values and views as strength the difference we all bring to the workplace. We encourage our community members to participate in our FAS Diversity Dialogues (https://hr.fas.harvard.edu/fas-diversity-dialogues) .

Department:Stem Cell and Regenerative BiologyPre-Employment Screening:Identity Schedule:Monday thru Friday 9am to 5pm

Some Holiday and Weekend Coverage may be Required Job Function:Research Job-Specific Responsibilities:

More specifically, job responsibilities include:

· Under direction, carry out a variety of routine laboratory protocols that include molecular biology techniques (e.g., molecular cloning, plasmid preps, RNA preps, and PCR analysis), protein analysis (Western blot, ELISA, FACS) and mammalian cell culture;

· Maintain mouse colony; carry out husbandry, genotype mouse strains, perform tail bleeding and injections for experiments;

· Assist with animal experiments that include surgeries and/or tissue harvests;

· Prepare and maintain stocks of reagents, solutions, and supplies;

· Assist with equipment maintenance and ordering of common lab supplies;

· Record experimental results and maintain accurate lab records.

School/Unit:Faculty of Arts and Sciences EEO Statement:We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions, or any other characteristic protected by law.Job Summary:

The Wagers laboratory studies the biology and function of adult tissue-specific stem cells, particularly those stem cells that form the skeletal muscle and blood. Current topics under investigation include regulation of stem cell function in normal tissue and under abnormal conditions such as aging and cancer. The lab is seeking a highly motivated candidate to assist with ongoing experiments to ascertain the role of specific blood-borne factors as well as genetic/epigenetic regulators in driving the defects in organ function that typically accompany old age. In particular, the Research Assistant will work together with the PI and other research staff to maintain project-specific transgenic and aging mouse colonies, to perform mouse survival surgeries, tissue collection and analysis by flow cytometry, immunostaining and other bioassays, and to organize data and collate research results. The assistant will also perform a variety of basic and general lab support and clerical tasks, including ordering and stocking of supplies, maintenance of equipment and preparation and storage of shared reagents.

Work will be performed under specific direction of the PI and/or senior lab members, although it is expected that certain research-related tasks will be performed independently. The assistant will also participate in frequent team meetings involving lab members and external collaborators, and, under the direction of the PI and senior research staff, will participate in preparing and presenting research results and summaries. Strong organizational and communication skills (both written and oral) are essential.

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