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Harvard University Research Assistant II in Cambridge, Massachusetts

52170BRAuto req ID:52170BRJob Code:403112 Research Assistant II Lab Location:USA - MA - Boston Business Title:Research Assistant II – Biological Applications of DNA NanotechnologySub-Unit:Wyss Institute Salary Grade:053Time Status:Full-time Union:55 - Hvd Union Cler & Tech Workers Basic Qualifications:College background or equivalent work experience in Biology, Immunology, Chemistry, Molecular Biology, Engineering or related field. A minimum of one year of directly related work experience. Higher education may count toward years of experience.Additional Qualifications:

• Bachelor’s degree

• 2+ years of research experience, preferably including experience with cell culture, molecular biology protocols (e.g., PCR), biochemistry protocols (e.g., antibody conjugations, purifications), tissue processing, in situ staining protocols (IF, IHC, FISH), fluorescence microscopy, and/or image analysis

• Good laboratory practices, attention to detail

• Experience in planning experiments and adjusting protocols, organizing data, and maintenance of lab notebook

• Competence in time management and working independently to achieve specific research milestones¿

• Effective communication (oral and written) skills and management of interactions with multiple lab members

• Basic computer skills

Additional Information:

This is a one year term position with the possibility of renewal.

The mission of the Wyss Institute for Biologically Inspired Engineering at Harvard University is to transform human healthcare and the environment by emulating the way nature builds. Developed as an alliance between Harvard and other premier academic and clinical partner institutions, Institute faculty and staff collaborate in high-risk, fundamental research and science-driven technology development. A major focus of the Institute is to translate the technologies developed by its faculty and staff into commercial products and therapies through collaborations with clinical investigators and establishment of corporate alliances and launching startup companies. For more information, visit: http://wyss.harvard.edu/.

Harvard offers an outstanding benefits package including:

  • Time Off: 3 - 4 weeks paid vacation, paid holiday break, 12 paid sick days, 11.5 paid holidays, and 3 paid personal days per year.

  • Medical/Dental/Vision: We offer a variety of excellent medical plans, dental & vision plans, all coverage begins as of your start date.

  • Retirement: University-funded retirement plan contributions from 5% to 15% of eligible compensation, based on age and earnings with full vesting after 3 years of service.

  • Tuition Assistance Program: Competitive tuition assistance program, $40 per class at the Harvard Extension School and discounted options through participating Harvard grad schools.

  • Transportation: Harvard offers a 50% discounted MBTA pass as well as additional options to assist employees in their daily commute.

  • Wellness options: Harvard offers programs and classes at little or no cost, including stress management, massages, nutrition, meditation and complimentary health services.

  • Harvard access to athletic facilities, libraries, campus events and many discounts throughout metro Boston.

Department:Wyss InstitutePre-Employment Screening:Identity Schedule:

Monday through Friday, 9am - 5pm

Job Function:Research Job-Specific Responsibilities:In conjunction with senior lab members, the RA will follow existing or help develop new protocols, manage equipment, reagents, and data, and have an opportunity to grow and work independently upon training. The successful candidate will also have access to a range of professional development and collaboration opportunities at the Wyss, including opportunities to present their work, receive relevant training, and interact with students, postdocs, and faculty.School/Unit:Harvard Medical School EEO Statement:We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions, or any other characteristic protected by law.Job Summary:The Wyss Institute for Biologically Inspired Engineering at Harvard University is seeking a talented individual to work as a Research Assistant II in Peng Yin’s lab. The lab specializes in developing and applying DNA-based technology, including the application of DNA probes to transform biomolecular imaging and understand cell and tissue organization. Techniques developed there include highly multiplexed tissue imaging with DNA barcoded probes, super-resolution multiplexed DNA-based optical imaging methods, as well as complementary methods of recording macromolecular features in synthetic DNA for sequencing readout. Recently, the lab has developed a multiplexed DNA-based signal amplification method called Signal Amplification by Exchange Reaction (SABER) to enable high-throughput multiplexed tissue imaging. The successful candidate may work on a variety of these imaging projects, applying molecular biology and biochemistry protocols to construct, amplify, conjugate, or assemble components and probe sets (such as fluorescence in situ hybridization oligos or antibodies) and in turn apply them to recording or fluorescence imaging applications in vitro (e.g., proteins in solution) or on biological materials (e.g., live or fixed cells and tissues for IF, IHC, FISH protocols). Equipment may include advanced optical microscopes (e.g., fluorescence, TIRF, or confocal), Next Generation Sequencers, and a variety of cell and molecular biology equipment.

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